Pickering emulsion stabilized by Haematococcus pluvialis protein particles and its application in dumpling stuffing

In this study, the oil-in-water Pickering emulsions were prepared using Haematococcus Pluvialis protein (HPP) particles as an emulsifier by a simple one-step emulsification method. The internal oil phase was as high as 70 % due to the excellent emulsifying properties of HPP, and the average size of oil droplets in the emulsion was around 20 mu m. The emulsion prepared by 2.5 % HPP with the oil phase ratio of 70 % showed the best stability after 14 days of storage, and the emulsion could maintain stability at acidic condition, high ionic strength, low and high temperatures. However, all emulsion samples exhibited shear thinning phenomenon, and the higher HPP concentration and oil phase ratio led to greater G ' and G '' modulus. NMR relaxation results showed that high concentration HPP could limit the mobility of free water in the emulsion and improve the emulsion stability. The HPP-stabilized emulsion could inhibit the oxidation of oil phase during storage due to the DPPH and ABTS radical scavenging activity of astaxanthin (AST) in HPP. Finally, the nutritional microspheres based on HPP-stabilized emulsion showed good stability in traditional dumplings and could reduce the loss of AST and DHA in algae oil during the boiling of dumplings.

Automated summary

In this study, oil-in-water Pickering emulsions were prepared using Haematococcus Pluvialis protein (HPP) particles as an emulsifier. The emulsion showed high stability with an internal oil phase as high as 70% and average oil droplet size of 20 μm. The emulsion stabilized by 2.5% HPP and 70% oil phase ratio exhibited the best stability, even under acidic conditions, high ionic strength, and low or high temperatures. Additionally, the HPP-stabilized emulsion inhibited oil phase oxidation during storage due to the presence of astaxanthin (AST) in HPP.