Quantitative proteomic analysis of cattle-yak and yak longissimus thoracis provides insights into the differential mechanisms of meat quality

In this study, proteins of cattle-yak longissimus thoracis (CYLT) and yak longissimus thoracis (YLT) were compared using tandem mass tag-labeled quantitative proteomic analysis. A total of 157 proteins were screened as differentially abundant proteins (DAPs) derived from 1551 quantitative proteins. Bioinformatics analysis revealed that the upregulated DAPs in CYLT were mainly involved in energy metabolism, oxidative stress, muscle fiber structure, and extracellular matrix (ECM), while the downregulated DAPs were mainly involved in energy metabolism and ECM function. The upregulated myoglobin, downregulation of NADH dehydrogenase, and upregulation of cytochrome oxidase indicated that CYLT initiates compensatory regulation in response to hypoxic high-altitude environments. Two differentially abundant myosins and five collagens suggested that CYLT and YLT may have distinct differences in the assembly structure of muscle fibers and connective tissue. These differences in energy metabolism and muscle structure will inevitably affect the postmortem physiology of muscle to meat and consequently the meat qualities. Therefore, our results will provide important clues to gain insight into the potential causes of meat quality differences between cattle-yak and yak based on high-altitude response.

Automated summary

By comparing the proteins of cattle-yak longissimus thoracis (CYLT) and yak longissimus thoracis (YLT), this study revealed differentially abundant proteins (DAPs) involved in energy metabolism, oxidative stress, muscle fiber structure, and extracellular matrix (ECM). The compensatory regulation of CYLT in response to hypoxic high-altitude environments was indicated by the upregulation of myoglobin, downregulation of NADH dehydrogenase, and upregulation of cytochrome oxidase. Furthermore, differences in muscle fiber assembly structure and connective tissue between CYLT and YLT were observed, which may affect postmortem muscle physiology and meat quality.