Genetic response of Salmonella Typhimurium to trans-cinnamaldehyde assisted heat treatment and its correlation with bacterial resistance in different low moisture food components

Our previous study found that water activity (aw)-and matrix-dependent bacterial resistance wasdeveloped in Salmonella Typhimurium during antimicrobial-assisted heat treatment in low moisture foods (LMFs) matrices. To better understand the molecular mechanism behind the observed bacterial resistance, gene expression analysis was conducted on S. Typhimurium adapted to different conditions with or without the trans-cinnamaldehyde (CA)-assisted heat treatment via quantitative polymerase chain reaction (qPCR). Expression profiles of nine stress-related genes were analyzed. The upregulation of rpoH and dnaK and downregulation of ompC were observed during bacterial adaptation in LMF matrices and the combined heat treatment, which likely contributed to the bacterial resistance during the combined treatment. Their expression profiles were partially consistent with the previously-observed effect of aw or matrix on bacterial resistance. The upregulation of rpoE, otsB, proV, and fadA was also observed during adaptation in LMF matrices and might contribute to desiccation resistance, but likely did not contribute to bacterial resistance during the combined heat treatment. The observed upre-gulation of fabA and downregulation of ibpA could not be directly linked to bacterial resistance to either desiccation or the combined heat treatment. The results may assist the development of more efficient processing methods against S. Typhimurium in LMFs.

Automated summary

Our study found that the resistance of Salmonella Typhimurium to antimicrobial-assisted heat treatment in low moisture foods (LMFs) is influenced by water activity (aw) and matrix. To understand the molecular mechanism behind this resistance, gene expression analysis was conducted. The upregulation of rpoH and dnaK and downregulation of ompC were observed, likely contributing to bacterial resistance during the combined treatment. The upregulation of rpoE, otsB, proV, and fadA might contribute to desiccation resistance, but not to bacterial resistance during the combined heat treatment. The results can aid in the development of more efficient processing methods against Salmonella Typhimurium in LMFs.