Valorisation of pork by-products to obtain antioxidant and antihypertensive peptides

The porcine liver could be used for the extraction of zinc-protoporphyrin (ZnPP) as a natural red meat pigment. During the autolysis process, porcine liver homogenates was incubated at pH 4.8 and 45 degrees C under anaerobic conditions to obtain insoluble ZnPP. After incubation, the homogenates were readjusted at pH 4.8, and at pH 7.5 before being centrifuged at 5500 x g for 20 min at 4 degrees C and the resulting supernatant were compared with the obtained at pH 4.8 at the beginning of the incubation. The molecular weight distributions of the porcine liver fractions at both pHs were very similar, however, eight essential amino acids were more abundant in fractions obtained at pH 4.8. Regarding the ORAC assay, porcine liver protein fraction at pH 4.8 showed the highest antioxidant capacity but antihypertensive inhibition was similar for both pHs. Peptides with strong bioactivity potential from aldehyde dehydrogenase, lactoylglutathione lyase, SEC14-like protein 3 and others were identi-fied. The findings have demonstrated the potential of the porcine liver to extract natural pigments and bioactive peptides.

Automated summary

Porcine liver can be used for extracting zinc-protoporphyrin (ZnPP) as a natural red meat pigment. In this study, porcine liver homogenates were incubated at pH 4.8 and 45 degrees C under anaerobic conditions to obtain insoluble ZnPP. The molecular weight distributions of the porcine liver fractions at pH 4.8 and pH 7.5 were similar, but the fractions obtained at pH 4.8 had higher abundance of eight essential amino acids. The results demonstrate the potential of porcine liver in extracting natural pigments and bioactive peptides.