Plant growth-promoting bacteria as affected by N availability as a suitable strategy to enhance the nutritional composition of lamb's lettuce affected by global warming

Heat and nutritional stresses have a significantly effect on the accumulation of bioactive and other compounds harmful to human health, like nitrates, in green leafy vegetables like lamb's lettuce. Plant growth-promoting bacteria (PGPB) have shown to confer beneficial biochemical changes to various crops under different stresses. The hypothesis proposed here is that the combination of optimal N level (2.5 Mm, 12 mM or 20 mM of N) with the inoculation of PGPB in plants exposed to heat shock (43 degrees C) may be a good strategy to obtain healthier lamb's lettuce with a higher yield. Results showed that a dose of 20 mM N can be considered as overfertilization. Moreover, the inoculation of plants fed with fertilizers with reduced N and under heat stress, resulted in higher productivity and content of sugars (60 %), amino acids (94 %), nitrogen (21 %), and total phenolic compounds (30 %), and a reduced content of nitrates (27 %).

Automated summary

Heat and nutritional stresses have a significant impact on the accumulation of harmful compounds in green leafy vegetables. The inoculation of plant growth-promoting bacteria (PGPB) combined with optimal nitrogen (N) levels under heat shock conditions can result in healthier and higher yielding crops. The results showed that overfertilization with 20 mM N was not beneficial, but reducing N levels and inoculating plants under heat stress improved productivity and increased the content of beneficial compounds.