Journal
EXPERIMENTAL DERMATOLOGY
Volume -, Issue -, Pages -Publisher
WILEY
DOI: 10.1111/exd.14899
Keywords
autofluorescence; multiphoton microscopy; second harmonic generation; skin disease
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Non-invasive optical examination is vital in dermatology for diagnosis, management, and research. Multiphoton microscopy uses submicron technology to stimulate autofluorescence and provides insights into cellular structure, redox status, and collagen fibers in the skin. This review highlights the principles and application of multiphoton microscopy in studying various skin conditions.
Non-invasive optical examination plays a crucial role in various aspects of dermatology, such as diagnosis, management and research. Multiphoton microscopy uses a unique submicron technology to stimulate autofluorescence (AF), allowing for the observation of cellular structure, assessment of redox status and quantification of collagen fibres. This advanced imaging technique offers dermatologists novel insights into the skin's structure, positioning it as a promising 'stethoscope' for future development in the field. This review provides an overview of multiphoton microscopy's principles, technology and application in studying normal skin, tumour and inflammatory diseases, as well as collagen-related and pigmentary diseases.
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