Journal
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Volume 258, Issue -, Pages -Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2023.115543
Keywords
PI3K-AKT-mTOR pathway; Phosphatidylinositol 3-kinase; PI3K; mTOR dual inhibitor; 7-Azaindzaole; Hematological malignancies; Acute myeloid leukemia
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The PI3K-Akt-mTOR pathway is highly activated in human hematological malignancies and has shown promise as a potential target for acute myeloid leukemia (AML) therapy. In this study, we synthesized a series of 7-azaindazole derivatives as potent dual inhibitors of PI3K/mTOR, with compound FD274 showing excellent inhibitory activity. In vitro and in vivo studies demonstrated the potential of FD274 as a targeted anti-AML drug candidate.
PI3K-Akt-mTOR pathway is a highly activated signal transduction pathway in human hematological malignancies and has been validated as a promising target for acute myeloid leukemia (AML) therapy. Herein, we designed and synthesized a series of 7-azaindazole derivatives as potent PI3K/mTOR dual inhibitors based on our previously reported FD223. Among them, compound FD274 showed excellent dual PI3K/mTOR inhibitory activity, with IC50 values against PI3K & alpha;/8/& gamma;/& delta; and mTOR of 0.65 nM, 1.57 nM, 0.65 nM, 0.42 nM, and 2.03 nM, respectively, superior to compound FD223. Compared to the positive drug Dactolisib, FD274 exhibited significant anti-proliferation of AML cell lines (HL-60 and MOLM-16 with IC50 values of 0.092 & mu;M and 0.084 & mu;M, respectively) in vitro. Furthermore, FD274 demonstrated dose-dependent inhibition of tumor growth in the HL60 xenograft model in vivo, with 91% inhibition of tumor growth at an intraperitoneal injection dose of 10 mg/kg and no observable toxicity. All of these results suggest that FD274 has potential for further development as a promising PI3K/mTOR targeted anti-AML drug candidate.
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