Rebuttal to Correspondence on The Environmental Microbiology Minimum Information (EMMI) Guidelines: qPCR and dPCR Quality and Reporting for Environmental Microbiology

Summary: Real-time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) methods have revolutionized environmental microbiology by providing quantitative organism-specific data. However, many researchers in the field fail to report necessary controls and methods, impacting the reliability of their study results. By implementing systematic controls, efficient workflow, and critical reporting elements, data credibility in environmental microbiology research can be enhanced to support disease prevention efforts.