Potential contribution of chlorella vulgaris to carbon-nitrogen turnover in freshwater ecosystems after a great sandstorm event

Urban lakes represent important land-water and nature-human dual interfaces that promote the cycling of elements from terrestrials to sediments and consequently modulating the stabilization of regional climate. However, whether disturbances caused by extreme weather events can have substantial effects on carbon-nitrogen (C-N) cycling in these ecosystems are vague. To explore the impact of phytoplankton on the ecological retention time of C-N, two kinds of freshwater (natural and landscape) were collected and conducted a microcosm experiment using a freshwater algal species Chlorella vulgaris. Sandstorm events increased dissolved inorganic carbon in freshwater (65.55 +/- 3.09 and 39.46 +/- 2.51 mg center dot L-1 for samples from Jinyang and Nankai, respectively) and significantly affected the relevant pathways of photosynthesis in Chlorella vulgaris, including enhancing chlorophyll fluorescence (The effective quantum yield of PSII at the fifth day of incubation was 0.34 and 0.35 for Nankai and Jinyang, respectively), promoting the synthesis of sugars and inhibiting the synthesis of glycine and serine related proteins. Besides, carbon from plant biomass accumulation and cellular metabolism (fulvic acidlike, polyaromatic-type humic acid and polycarboxylate-type humic acid, etc.) was enriched into residues and become a kind of energy source for the decomposer (TC mass increased by 1.63-2.13 times after 21 days of incubation). This means that the accumulation and consumption of carbon and nitrogen in the residue can be used to track the processes controlling the long-term C-N cycle. Our findings shed light on the plant residues were key factors contributing to the formation of water carbon pool, breaks the traditional theory that dissolved carbonates cannot produce carbon sinks.

Automated summary

Urban lakes play a crucial role in the cycling of elements and stabilization of regional climate. However, the impacts of extreme weather events on carbon-nitrogen cycling in these ecosystems are unclear. A microcosm experiment using Chlorella vulgaris showed that sandstorm events increased dissolved inorganic carbon and affected photosynthesis pathways. Plant biomass accumulation and metabolism enriched carbon into residues, which served as an energy source for decomposers. This study provides insight into the formation of water carbon pools and challenges the traditional theory of dissolved carbonates as carbon sinks.