Journal
ENVIRONMENTAL POLLUTION
Volume 334, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2023.122199
Keywords
Triclosan; Zebrafish; Cardiotoxicity; beta-adrenergic receptor signaling pathway
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In this study, we investigated the toxic effects of triclosan (TCS) exposure on heart development in zebrafish embryos. TCS exposure caused significant cardiac dysfunction, oxidative damage, and abnormal apoptosis in heart cells. The upregulation of beta-adrenergic receptor (beta-AR) signaling pathway genes and downregulation of miR-133b were found to contribute to TCS-induced cardiotoxicity.
As a commonly used antibacterial agent in daily consumer products, triclosan (TCS) has attracted significant attention due to its potential environmental risks. In this study, we investigated the toxic effects of TCS exposure (1.4 mu M) on heart development in zebrafish embryos. Our findings revealed that TCS exposure caused significant cardiac dysfunction, characterized by pericardial edema, malformations in the heart structure, and a slow heart rate. Additionally, TCS exposure induced oxidative damage and abnormal apoptosis in heart cells through the upregulation of beta-adrenergic receptor (beta-AR) signaling pathway genes (adrb1, adrb2a, arrb2b), similar to the effects induced by beta-AR agonists. Notably, the adverse effects of TCS exposure were alleviated by beta-AR antagonists. Using high-throughput transcriptome miRNA sequencing and targeted miRNA screening, we focused on miR-133b, which targets adrb1 and was down-regulated by TCS exposure, as a potential contributor to TCS induced cardiotoxicity. Inhibition of miR-133b produced similar toxic effects as TCS exposure, while over expression of miR-133b down-regulated the beta-AR signaling pathway and rescued heart defects caused by TCS. In summary, our findings provide new insights into the mechanisms underlying the cardiotoxic effects of TCS. We suggest that targeting the beta-AR pathway and miR-133b may be effective strategies for pharmacotherapy in cardiotoxicity induced by environmental pollutants such as TCS.
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