Journal
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Volume 211, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2023.105367
Keywords
Amino acid; Ammonium transporter; Nitrogen assimilation; Nutrient; Phytohormone; Populus; Proteome
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Using physiological and proteomic assays, we found that N deficiency inhibits the growth, and N uptake and assimilation in poplar roots, as well as the levels of phytohormones such as abscisic acid and salicylic acid. On the other hand, high N stimulates these processes and leads to differentially abundant proteins in poplar roots. Our results suggest a correlation between proteomic reconfigurations and physiological alterations in poplars acclimating to changing N availability.
Although the roots of woody plants are essential for nitrogen (N) uptake and assimilation, proteomic regulation underlying these processes is unclear in acclimation to changing N availability. Here, we carried out physiological and proteomic assays in Populus x canescens sapling roots treated with one of low, normal and high levels of NH4NO3. N deficiency induced lower concentrations of NH4+, total N, amino acids, inhibited enzymatic activities of nitrate reductase and glutamate synthase, decreased levels of abscisic acid and salicylic acid in poplar roots, and high N had the opposite effects. There were 552 and 247 significantly differentially abundant proteins (DAPs) in low and high vs normal N-supplied poplar roots, respectively. Notably, the abundances of a few poplar proteins, including nitrite reductase 1, glutamate synthase 1, and glutamate dehydrogenase 1, corresponded well to N-induced physiological changes in poplar roots. Functional characterization of N-responsive poplar ammonium transporter 2;1 and glutamate dehydrogenase 2 suggests both play pivotal roles in N uptake and assimilation in response to altering N levels. These results suggest that the growth, N uptake and assimilation, and phytohormonal levels are inhibited by N deficiency, but stimulated by high N, and the proteomic reconfigurations occur underlying these physiological alterations in poplars in acclimation to changing N availability.
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