A novel tethered phenothiazine-coumarin fluorescence probe for rapid detection of hypochlorite ion and its applications

A novel tethered phenothiazine-coumarin fluorescent probe C-Ptz was synthesized for the detection of ClO- ion. Upon the reaction of C-Ptz with ClO- ions, a fluorescence enhancement (similar to 42 folds) was observed by blocking PET quenching pathway. The probe C-Ptz showed highly selectivity and fast turn-on response (within 3 s) towards ClO- with detection limit down to 12.6 nM. Furthermore, the fluorescent probe C-Ptz was utilized to the fluorescence imaging of ClO- in living cells. In addition, its measurement of ClO- was also performed in real-life water samples.

Automated summary

A novel fluorescent probe C-Ptz was synthesized for the detection of ClO- ion. The reaction of C-Ptz with ClO- ions resulted in a fluorescence enhancement (about 42 folds) by blocking PET quenching pathway. The probe C-Ptz showed highly selectivity and fast turn-on response (within 3 s) towards ClO- with detection limit down to 12.6 nM. Furthermore, the fluorescent probe C-Ptz was utilized for fluorescence imaging of ClO- in living cells and measurement of ClO- in real-life water samples.