Description of Flavimaribacter sediminis gen. nov., sp. nov., a New Member of the Family Rhizobiaceae Isolated from Marine Sediment

A novel Gram-staining-negative, aerobic and rod-shaped bacterium, designated WL0058(T), was isolated from coastal sediment sample collected in Nantong city, Jiangsu province of China (120 degrees 51' 13. E, 32 degrees 6' 26'' N) in October 2020. Strain WL0058(T) was found to grow at 4-37 degrees C (optimum, 28 degrees C) with 1.5-4.0% NaCl (optimum, 4.0%) and displayed alkaliphilic growth with the pH range of pH 6.0-10.0 (optimum, pH 6.0). Phylogenetic trees constructed based on 16S rRNA gene sequence indicated that strain WL0058(T) is a member of the family Rhizobiaceae, shared the highest similarity with Hoeflea prorocentri CCTCC AB 2016294(T) (97.7%) and constituted a sub-cluster within the family with it, while the similarity with others in the family Rhizobiaceae was lower than 97.0%. The G + C content of genomic DNA was 59.5 mol%. Polar lipids profile of strain WL0058(T) included phosphatidylcholine (PC), phosphatidylethanolamine (PE), and glycolipid (GL), phosphatidylmonomethylethanolamine (PME) and two unidentified polar lipids (L). The major isoprenoid quinone was determined to be Q-10 and the major fatty acids were C-16:0, C-18:0, summed features 4 (iso-C-17:1 and/or anteiso-C-17:1), and summed features 8 (C-18:1.omega 6c and/or C-18:1.7c). As inferred from the morphology, physiology, and biochemical analysis, genotypic characteristics, and the phylogenetic trees, strain WL0058(T) ought to be recognized as a novel genus in the family Rhizobiaceae, for which the name Flavimaribacter sediminis gen. nov., sp. nov. The type strain of Flavimaribacter sediminis gen. nov., sp. nov. is WL0058(T) (= MCCC 1K06063(T) = JCM 34659(T) = GDMCC 1.2448(T)).

Automated summary

A novel Gram-staining-negative, aerobic and rod-shaped bacterium WL0058(T) was isolated from coastal sediment sample in Nantong, China. It displayed adaptability to temperature, salinity, and alkalinity. Based on morphology, physiology, biochemical analysis, genotypic characteristics, and phylogenetic trees, strain WL0058(T) should be recognized as a new genus Flavimaribacter sediminis gen. nov., sp. nov.