Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

Food safety problems have become one of the most important public health issues worldwide. Therefore, the development of rapid, effective and robust detection is of great importance. Amongst a range of methods, nucleic acid isothermal amplification (NAIA) plays a great role in food safety detection. However, the widespread application remains limited due to a few shortcomings. CRISPR/ Cas system has emerged as a powerful tool in nucleic acid detection, which could be readily integrated with NAIA to improve the detection sensitivity, specificity, adaptability versatility and dependability. However, currently there was a lack of a comprehensive summary regarding the integration of NAIA and CRISPR/Cas in the field of food safety detection. In this review, the recent advances in food safety detection based on CRISPR/Cas-integrated NAIA were comprehensively reviewed. To begin with, the development of NAIA was summarized. Then, the types and working principles of CRISPR/Cas were introduced. The applications of the integration of NAIA and CRISPR/ Cas for food safety were mainly introduced and objectively discussed. Lastly, current challenges and future opportunities were proposed. In summary, this technology is expected to become an important approach for food safety detection, leading to a safer and more reliable food industry.

Automated summary

Food safety issues are of utmost importance in public health worldwide, and the development of rapid and effective detection methods is crucial. Nucleic acid isothermal amplification (NAIA) plays a significant role in food safety detection, and the integration of NAIA with the CRISPR/Cas system offers improved sensitivity, specificity, adaptability, versatility, and dependability. However, there is currently a lack of comprehensive summaries on the integration of NAIA and CRISPR/Cas in the field of food safety detection. This review comprehensively examines recent advances in CRISPR/Cas-integrated NAIA for food safety detection and discusses current challenges and future opportunities.