Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 230, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2023.113502
Keywords
Composite nanoparticle; Calcium phosphate; Basic fibroblast growth factor; Iron oxide nanocrystal; Heparin; Cell proliferation
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In this study, biocompatible calcium phosphate nanoparticles were successfully fabricated as a multifunctional delivery carrier of basic fibroblast growth factor (bFGF). These nanoparticles co-immobilized bFGF, heparin, and ferucarbotran, and showed stable dispersion in water. They released bFGF in a dose-dependent manner and promoted or inhibited cell proliferation depending on the cell type and dose.
Basic fibroblast growth factor (bFGF) is a therapeutic protein that can enhance angiogenesis, wound healing, and tissue regeneration; however, it is extremely unstable even under a normal physiological environment. Biocompatible calcium phosphate (CaP) nanoparticles (NPs) co-immobilizing bFGF, heparin, and ferucarbotran would be useful as a multifunctional delivery carrier of bFGF. In this study, such NPs were successfully fabricated by a coprecipitation process, using a labile supersaturated CaP solution containing bFGF, heparin, and ferucarbotran. The NPs showed relatively high negative zeta potential (-12 mV) because of the negatively charged heparin, which enabled their stable dispersion in water. The hydrodynamic diameter of the NPs was around 200 nm. Immunoreactive bFGF was released from the NPs in an acellular medium dose-dependently. The NPs promoted proliferation of baby hamster kidney fibroblasts (BHK-21 cells) and mouse osteoblastic MC3T3-E1 cells at a certain dose range, although they inhibited proliferation of rat pheochromocytoma (PC-12) cells. These results demonstrated that the effect of the NPs on cell proliferation was dependent on the cell type and dose, the details of which should be investigated in a future study.
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