Biosynthesis of gold nanoparticles using jellyfish nematocyst venom protein and evaluation of their anticancer activity against breast cancer cells: In-vitro study

In the current study, biosynthesis of gold nanoparticles was carried out using jellyfish nematocyst venom protein extracted from phosphate-buffered saline (PBS) and double distilled water (DDW). The results of the two different gold nanoparticles reported with spherical morphology with a size range of 31 nm and 27 nm respectively. The toxicity of gold nanoparticles was analyzed using normal Human embryonic kidney 293 (HEK293) cell line by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. In addition, the anti-cancer activity of venom protein, PBS/DDW extracted venom protein-mediated synthesized gold nanoparticles, and standard drug 5-Fluorouracil was evaluated using Michigan Cancer Foundation-7 (MCF-7) cancer cell line.

Automated summary

The study synthesized gold nanoparticles using jellyfish nematocyst venom protein extracted from PBS and DDW. The two different nanoparticles exhibited spherical morphology with sizes ranging from 31 nm to 27 nm respectively. The toxicity of the nanoparticles was analyzed using HEK293 cells through the MTT assay. Moreover, the anti-cancer activity of the venom protein, PBS/DDW-extracted venom protein-mediated synthesized gold nanoparticles, and standard drug 5-Fluorouracil was evaluated using MCF-7 cancer cells.