Glutathione-templated blue emitting copper nanoclusters as selective fluorescent probe for quantification of nitrofurazone

In this work, copper nanoclusters (Cu NCs) were successfully synthesized through one-pot strategy by protecting of glutathione (GSH) and reducing of ascorbic acid (AA). The probe displayed excellent comprehensive stability and realized the detection of nitrofurazone. Fluorescence of GSH-Cu NCs was quenched by nitrofurazone with good linear relationship of nitrofurazone concentration from 0.5 to 100 mu M. Significantly, GSH-Cu NCs showed superior selectivity of nitrofurazone on account of static quenching and inner filter effect (IFE) mechanism. More importantly, recovery experiments of nitrofurazone detection obtained satisfactory results in bovine serum. The sensor provided better detection platform of nitrofurazone with superior fluorescence properties.

Automated summary

In this study, copper nanoclusters (Cu NCs) were synthesized successfully through a one-pot strategy by protecting glutathione (GSH) and reducing ascorbic acid (AA). The probe showed excellent stability and was able to detect nitrofurazone. The fluorescence of GSH-Cu NCs was quenched by nitrofurazone, and there was a good linear relationship between the concentration of nitrofurazone and the quenching. GSH-Cu NCs demonstrated superior selectivity towards nitrofurazone due to static quenching and inner filter effect (IFE) mechanism. Furthermore, satisfactory results were obtained for nitrofurazone detection in bovine serum. The sensor provided a better detection platform for nitrofurazone with superior fluorescence properties.