Characterization of a novel endo-1,3-fucanase from marine bacterium Wenyingzhuangia fucanilytica reveals the presence of diversity within glycoside hydrolase family 168

Article Chemistry, Applied

Characterization of an endo-1,3-fucanase from marine bacterium Wenyingzhuangia aestuarii: The first member of a novel glycoside hydrolase family GH174

Guanchen Liu et al.

Summary: A new bacterial enzyme, Fun174A, was discovered to degrade sulfated fucans in sea cucumbers, exhibiting significant biological and biomedical activities.

CARBOHYDRATE POLYMERS (2023)

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Article Biotechnology & Applied Microbiology

SignalP 6.0 predicts all five types of signal peptides using protein language models

Felix Teufel et al.

Summary: Signal peptides are short amino acid sequences that regulate protein secretion and translocation. SignalP 6.0, a machine learning model, is introduced to detect all types of signal peptides, including those applicable to metagenomic data.

NATURE BIOTECHNOLOGY (2022)

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Article Biochemistry & Molecular Biology

In-depth structural characterization of oligosaccharides released by GH107 endofucanase MfFcnA reveals enzyme subsite specificity and sulfated fucan substructural features

David Ropartz et al.

Summary: The extracellular matrix of brown algae is a rich source of fucose-containing sulfated polysaccharides (FCSPs). In this study, researchers used an enzyme called MfFcnA to deconstruct the structure of sulfated fucans from Pelvetia canaliculata, a type of brown algae. They found that the structure of these sulfated fucans is more complex than initially expected, with diverse species and branching structures. This study highlights the potential for MfFcnA to produce structurally diverse oligofucan products that could be screened for bioactivities.

GLYCOBIOLOGY (2022)

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Article Chemistry, Applied

Utilizing heterologously overexpressed endo-1,3-fucanase to investigate the structure of sulfated fucan from sea cucumber (Holothuria hilla)

Guangning Chen et al.

Summary: This study introduced the application of a heterologously overexpressed endo-1,3-fucanase in the structural identification of sea cucumber sulfated fucan. The results demonstrated the cost-effective and efficient preparation of the enzyme, providing superior oligosaccharide recovery for structural investigations. The identified major structure of sulfated fucan from sea cucumber highlighted the superiority of the heterologously overexpressed fucanase over wild-type enzyme.

CARBOHYDRATE POLYMERS (2021)

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Article Chemistry, Applied

Overexpression and biochemical characterization of a truncated endo-α (1 → 3)-fucoidanase from alteromonas sp. SN-1009

Chenlu Zhu et al.

Summary: A novel endo-alpha (1->3)-fucoidanase was characterized, showing high activity under specific conditions. Analysis of its degradation products confirmed its significant role in fucoidan degradation.

FOOD CHEMISTRY (2021)

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Article Biochemistry & Molecular Biology

Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation

Ivica Letunic et al.

Summary: The Interactive Tree Of Life is an online tool for displaying, manipulating, and annotating phylogenetic and other trees. The latest version 5 introduces a new tree display engine and several new features, such as a new dataset type and expanded annotation options. Users can now also manually annotate trees by hand, providing more flexibility and control.

NUCLEIC ACIDS RESEARCH (2021)

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Article Microbiology