Journal
CARBOHYDRATE POLYMERS
Volume 312, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2023.120796
Keywords
Chitosan; N -functionalized biopolymer; Antimicrobial activity; Structure -activity relationship
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In order to address the issues of antibiotic resistance and toxicity of synthetic polymers, we developed a synthetic method to create biocompatible polymers with broad spectrum antimicrobial properties. By incorporating cationic and hydrophobic functionality into N-functionalized chitosan polymers, we achieved optimal antibacterial effects against four bacterial strains. The polymer showed stronger inhibition and killing effects on Gram positive bacteria compared to Gram negative bacteria, as confirmed by growth kinetics and SEM imaging.
To overcome the problem of antibiotic resistance and toxicity of synthetic polymers, herein we report the synthesis of biocompatible polymers which can serve as broad spectrum antimicrobials. A regioselective synthetic method was developed to synthesize N-functionalized chitosan polymers having similar degree of substitution of cationic and hydrophobic functionality with different lipophilic chains. We obtained optimum antibacterial effect by utilizing the combination of cationic and longer lipophilic chain in the polymer, against four bacterial strains. Inhibition and killing of bacteria were more pronounced in Gram positive bacteria than in Gram negative bacteria. Growth kinetics and scanning electron microscopy imaging of the polymer treated bacterial cells confirmed the inhibition of bacterial growth, morphological changes in the structure and membrane disruption in the cells as compared to the growth control for each strain. Further investigation into the toxicity and selectivity of the polymers guided us to develop a structure-activity relationship for this class of biocompatible polymers.
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