4.7 Article

Programming the quorum sensing-based AND gate in Shewanella oneidensis for logic gated-microbial fuel cells

Journal

CHEMICAL COMMUNICATIONS
Volume 51, Issue 20, Pages 4184-4187

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc00026b

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Funding

  1. Chinese National High Technology Research and Development Program (863'' Program) [2012AA02A701]
  2. National Basic Research Program of China (973'' Program) [2014CB745100]
  3. National Natural Science Foundation of China (NSFC) [21376174]
  4. Singapore Centre on Environmental Life Sciences Engineering (Singapore)

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An AND logic gate based on a synthetic quorum-sensing (QS) module was constructed in a Shewanella oneidensis MR-1 mtrA knockout mutant. The presence of two input signals activated the expression of a periplasmic decaheme cytochrome MtrA to regenerate the extracellular electron transfer conduit, enabling the construction of AND-gated microbial fuel cells.

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