Journal
BRAZILIAN JOURNAL OF MICROBIOLOGY
Volume -, Issue -, Pages -Publisher
SPRINGER
DOI: 10.1007/s42770-023-01037-8
Keywords
Biocontrol; Klebsiella variicola; Klebsiella pneumoniae complex; Multidrug resistance; Phage therapy; Zebrafish
Categories
Ask authors/readers for more resources
A novel phage (KPP-1) infecting Klebsiella variicola was isolated and characterized from a natural water stream. The phage showed inhibitory effects on the growth of K. variicola both in vitro and in vivo. The study suggests that KPP-1 has the potential to be developed as a biocontrol agent against multidrug-resistant K. variicola.
Klebsiella variicola strain was identified from a natural water stream. Novel phage (KPP-1) infecting K. variicola was isolated and characterized. The biocontrol efficacy of KPP-1 against K. variicola-infected adult zebrafish was also investigated. The host K. variicola strain was resistant to six of the antibiotics tested and comprised the virulence genes kfuBC, fim, ureA, and Wza-Wzb-Wzc(cps). Morphological analysis by transmission electron microscopy revealed that KPP-1 has icosahedron head and tail structures. The latent period and burst size of KPP-1 were 20 min and 88 PFU per infected cell, respectively, at a multiplicity of infection of 0.1. KPP-1 was stable over a broad pH range (3-11), temperature (4-50 & DEG;C), and salinity (0.1-3%). KPP-1 inhibits the growth of K. variicola in vitro and in vivo. In the zebrafish infection model, treatment with KPP-1-infected K. variicola demonstrated 56% of cumulative survival. This suggests the possibility of developing KPP-1 as a potential biocontrol agent against multidrug-resistant K. variicola that belongs to the K. pneumoniae complex.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available