4.6 Article

Improving phytase production in Pichia pastoris fermentations through de-repression and methanol induction optimization

Related references

Note: Only part of the references are listed.
Article Biotechnology & Applied Microbiology

Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Laura Navone et al.

Summary: The study explores potential bottlenecks in expressing E. coli AppA phytase in P. pastoris by using bidirectional promoters (BDPs) and other proteins. Transcriptional studies provide insights into the expression profile of BDPs. The use of ERV2, a flavoprotein not previously characterized in P. pastoris, improves phytase expression, suggesting it as an alternative pathway to ERO1.

MICROBIAL CELL FACTORIES (2021)

Article Biotechnology & Applied Microbiology

Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Laura Navone et al.

Summary: This study presents a thermostable variant of the E. coli AppA phytase, ApV1, with an extra non-consecutive disulfide bond, which shows promising thermostability but leads to decreased production in Pichia pastoris. Co-expression of folding chaperones like protein disulfide bond isomerase (Pdi) significantly increased the production of ApV1 and restored yields to levels comparable with the wild-type enzyme. Engineering for enhanced enzymatic properties may result in folding complexity and reduced production in microbial systems, highlighting the importance of developing improved production strains in parallel to achieve desirable levels of recombinant protein for industrial processes.

BIOTECHNOLOGY FOR BIOFUELS (2021)

Article Biotechnology & Applied Microbiology

A multistrategy approach for improving the expression ofE. coliphytase inPichia pastoris

Yuankun Helian et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

A rational design to enhance the resistance of Escherichia coli phytase appA to trypsin

Xi Wang et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2018)

Article Multidisciplinary Sciences

Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

Thomas Vogl et al.

NATURE COMMUNICATIONS (2018)

Article Biochemistry & Molecular Biology

Bioprocess for the production of recombinant HAP phytase of the thermophilic mold Sporotrichum thermophile and its structural and biochemical characteristics

Anay Kumar Maurya et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2017)

Article Biochemistry & Molecular Biology

Temperature stability of proteins: Analysis of irreversible denaturation using isothermal calorimetry

Arne Schon et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2017)

Article Multidisciplinary Sciences

Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating

Stanislav Mazurenko et al.

SCIENTIFIC REPORTS (2017)

Article Biotechnology & Applied Microbiology

Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution

Amol V. Shivange et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2016)

Article Biotechnology & Applied Microbiology

Novel Glucose-1-Phosphatase with High Phytase Activity and Unusual Metal Ion Activation from Soil Bacterium Pantoea sp Strain 3.5.1

Aliya D. Suleimanova et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2015)

Article Biotechnology & Applied Microbiology

Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

Cheng Li et al.

BMC BIOTECHNOLOGY (2015)

Article Chemistry, Multidisciplinary

Enzymology and thermal stability of phytase appA mutants

Xi Wang et al.

RSC ADVANCES (2015)

Article Biochemistry & Molecular Biology

Enhancement of Thermostability and Kinetic Efficiency of Aspergillus niger PhyA Phytase by Site-Directed Mutagenesis

Ardeshir Hesampour et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2015)

Review Agriculture, Multidisciplinary

Phytase in non-ruminant animal nutrition: a critical review on phytase activities in the gastrointestinal tract and influencing factors

Yueming Dersjant-Li et al.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2015)

Article Biochemical Research Methods

In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response

Corinna Rebnegger et al.

BIOTECHNOLOGY JOURNAL (2014)

Article Biotechnology & Applied Microbiology

Multi-site saturation by OmniChange yields a pH- and thermally improved phytase

Amol V. Shivange et al.

JOURNAL OF BIOTECHNOLOGY (2014)

Article Agriculture, Dairy & Animal Science

Feed enzymes: The science, practice, and metabolic realities

V. Ravindran

JOURNAL OF APPLIED POULTRY RESEARCH (2013)

Article Biotechnology & Applied Microbiology

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

Roland Prielhofer et al.

MICROBIAL CELL FACTORIES (2013)

Article Biotechnology & Applied Microbiology

Directed evolution of a highly active Yersinia mollaretii phytase

Amol V. Shivange et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2012)

Review Biotechnology & Applied Microbiology

Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: A review

Gabriel Potvin et al.

BIOCHEMICAL ENGINEERING JOURNAL (2012)

Article Biotechnology & Applied Microbiology

Control of specific growth rate to enhance the production of a novel disintegrin, saxatilin, in recombinant Pichia pastoris

Cheol-Ki Min et al.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2010)

Article Biotechnology & Applied Microbiology

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Pieter P. Jacobs et al.

MICROBIAL CELL FACTORIES (2010)

Article Biochemistry & Molecular Biology

Interrelation ships of glycosylation and aggregation kinetics for Peniophora lycii phytase

R Hoiberg-Nielsen et al.

BIOCHEMISTRY (2006)

Article Biotechnology & Applied Microbiology

Model-based specific growth rate control for Pichia pastoris to improve recombinant protein production

HT Ren et al.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2005)

Article Biotechnology & Applied Microbiology

A Pichia pastoris fermentation strategy for enhancing the heterologous expression of an Escherichia coli phytase

CC Chen et al.

ENZYME AND MICROBIAL TECHNOLOGY (2004)

Review Biochemical Research Methods

Dealing with complexity: evolutionary engineering and genome shuffling

R Petri et al.

CURRENT OPINION IN BIOTECHNOLOGY (2004)

Article Biotechnology & Applied Microbiology

Scale-up of a high cell density continuous culture with Pichia pastoris X-33 for the constitutive expression of rh-chitinase

BM Schilling et al.

BIOTECHNOLOGY PROGRESS (2001)

Article Biochemistry & Molecular Biology

Exchanging the active site between phytases for altering the functional properties of the enzyme

M Lehmann et al.

PROTEIN SCIENCE (2000)