A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines

Recombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor & alpha; with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research. This study describes a method of generating recombinant rabbit monoclonal antibodies by combining rabbit immunization, cell hybridization, antibody gene isolation and a sequence-based approach to prioritizing antibodies for testing. Rabbit monoclonal antibodies generated against mouse soluble IL-6 receptor & alpha; were validated in Bio-Plex. Their affinity reached 10-9 to 10-12 M, as measured using a surface plasmon resonance technology.

Automated summary

This study presents a convenient and cost-effective method for generating recombinant rabbit monoclonal antibodies (rabbit rAbs). The method combines rabbit immunization, cell hybridization, antibody gene isolation, and a sequence-based approach. The generated rabbit rAbs showed high affinity for mouse soluble IL-6 receptor α.