Journal
BIOSENSORS & BIOELECTRONICS
Volume 235, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2023.115398
Keywords
Hollow heterostructured nanocubes; Nanozyme; Synergistic amplification; Lung cancer biomarker; Photoelectrochemical biosensor
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In this study, hollow SnO2/CdS QDs/CdCO3 heterostructured nanocubes were synthesized and used to construct a sandwich-typed photoelectrochemical (PEC) immunosensor for the detection of CYFRA 21-1. The PEC responses were amplified by the in-situ catalytic precipitation catalyzed by PtPd/MnCo-CeO2 nanozyme. The biosensor showed a wide linear range and a low limit of detection, making it suitable for detecting cancer biomarkers in clinic.
Nowadays, lung cancer is one of the most dangerous cancers threatening human life all over the world. As a crucial biomarker, cytokeratin 19 fragment 21-1 (CYFRA 21-1) is extraordinary important for diagnosis of nonsmall cell lung cancer (NSCLC). In this work, we synthesized hollow SnO2/CdS QDs/CdCO3 heterostructured nanocubes with high and stable photocurrents, which applied to construction of a sandwich-typed photoelectrochemical (PEC) immunosensor for detection of CYFRA 21-1, integrated by in-situ catalytic precipitation strategy with home-built PtPd alloy anchored MnCo-CeO2 (PtPd/MnCo-CeO2) nanozyme for synergistic amplification. The interfacial electron transfer mechanism upon visible-light irradiation was investigated in details. Further, the PEC responses were seriously quenched by the specific immunoreaction and precipitation catalyzed by the PtPd/MnCo-CeO2 nanozyme. The established biosensor showed a wider linear range of 0.001-200 ng mL-1 and a lower limit of detection (LOD = 0.2 pg mL-1, S/N = 3), coupled by exploring such analysis even in diluted human serum sample. This work opens a constructive avenue to develop ultrasensitive PEC sensing platforms for detecting diverse cancer biomarkers in clinic.
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