Journal
BIOSENSORS & BIOELECTRONICS
Volume 237, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2023.115557
Keywords
Self-powered dual-mode sensing strategy; DNA nanoring; Malignant tumor biomarke; High-sensitivity detection
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MicroRNA-21 (miRNA-21) is an oncogenic miRNA that is upregulated in almost all malignant tumors and has a broad spectrum of tumor recognition characteristics. A new dual-mode self-powered sensing platform using Au nanoparticles/graphdiyne as the electrode substrate and DNA nanoring for highly sensitive and specific detection of miRNA-21 is developed in this study. This strategy provides a new reference for the sensitive detection of microRNA and has immense potential for application in the screening and detection of clinical nucleic acid diseases.
MicroRNA-21 (miRNA-21) is currently the only known oncogenic miRNA that is upregulated in almost all malignant tumors and exhibits a broad spectrum of tumor recognition characteristics. It holds significant value in the early diagnosis, malignant degree assessment, and prognostic evaluation of tumors. In this study, a novel dual-mode self-powered sensing platform is developed using Au nanoparticles/graphdiyne as the electrode substrate and combined with DNA nanoring for highly sensitive and specific detection of miRNA-21. The DNA nanoring structure, which is easy to prepare and contains multiple recognition sites, induces significant electrochemical/colorimetric signal responses of the signaling molecule methylene blue. Under optimal conditions, the linear ranges of the electrochemical and colorimetric detection modes of this self-powered sensor are 0.1 fM100 pM and 0.1 fM-10 nM, respectively, with the detection limits of 35.1 aM and 61.6 aM (S/N=3). This strategy provides a new reference for the sensitive detection of microRNA and has immense potential for application in the screening and detection of clinical nucleic acid diseases.
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