Biological carbon promotes the recovery of anammox granular sludge after starvation

This article adopts the strategy of adding biochar and increasing HRT to accelerate the performance and particle morphology recovery of anaerobic ammonia oxidation granular sludge stored at room temperature for 68 days. The results showed that biochar accelerated the death of heterotrophic bacteria, shortened the cell lysis and lag period of the recovery process by 4 days, and it only took 28 days for the nitrogen removal performance of the reactor to recover to the original level, and 56 days for re-granulation. Biochar promoted the secretion of EPS (56.96 mg gVSS-1), and the sludge volume and nitrogen removal performance of the bioreactor remain stable. Biochar also accelerated the growth of Anammox bacteria. The abundance of Anammox bacteria in the biochar reactor reached 38.76% on the 28th day. The high abundance of functional bacteria and the optimized community structure of biochar made system (Candidatus_Kuenenia: 38.30%) more risk-resistant than control reactor.

Automated summary

This article explores the use of biochar and increased HRT to enhance the recovery of stored anaerobic ammonia oxidation granular sludge. The results show that biochar accelerates the recovery process, shortening the time needed for nitrogen removal and re-granulation. It also promotes the growth of Anammox bacteria and improves the stability of the bioreactor. The optimized community structure of biochar makes the system more resistant to risks.