Role of pretreatment type and microbial mechanisms on enhancing volatile fatty acids production during anaerobic fermentation of refinery waste activated sludge

This study compared the effects of alkaline, thermal, thermal-peroxymonosulfate (PMS), and alkyl polyglucose (APG) pretreatments on volatile fatty acids (VFAs) production from refinery waste activated sludge (RWAS), including VFAs yield, composition, organics components, microbial communities, and the potential improvement of mechanisms. All pretreatments effectively enhanced the bioconversion of RWAS and consequently promoted the hydrolysis process, which inhibited the methanogenesis process. However, the release of lignin/carboxyl-rich alicyclic molecules (CRAM)-like compounds and tannin substances in Thermal-PMS and APG groups significantly influenced the acidogenesis and acetogenesis processes. Among all pretreatments, alkaline pretreatment showed the highest VFAs yield of 95.06 mg/g volatile solids (VS) and VS removal of 17%. This result could be associated with the enrichment of functional hydrolytic-acidification bacteria, such as Planococcus and Soehngenia, and increased metabolism of amino acids, carbohydrates, and nucleotides. By considering an economical and efficient perspective, this study recommended the alkaline pretreatment for the anaerobic fermentation of RWAS.

Automated summary

This study compared the effects of different pretreatments on VFAs production from RWAS, and found that alkaline pretreatment showed the highest VFAs yield. The release of CRAM-like compounds and tannin substances in Thermal-PMS and APG groups significantly influenced the acidogenesis and acetogenesis processes. Economically and efficiently, alkaline pretreatment is recommended for the anaerobic fermentation of RWAS.