4.5 Article

Computer-aided design of novel cellobiose 2-epimerase for efficient synthesis of lactulose using lactose

Related references

Note: Only part of the references are listed.
Article Chemistry, Multidisciplinary

Gene Fusion and Directed Evolution to Break Structural Symmetry and Boost Catalysis by an Oligomeric C-C Bond-Forming Enzyme

Guangcai Xu et al.

Summary: This study used gene duplication and fusion strategy to evolve a promiscuous homohexameric enzyme into an efficient catalyst with high catalytic activity for enantioselective reactions. Through directed evolution and structural analysis, the asymmetric features of the protein were revealed, suggesting that this strategy may become an important technique in enzyme engineering.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022)

Article Biochemistry & Molecular Biology

AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models

Mihaly Varadi et al.

Summary: AlphaFold DB is an openly accessible database with high-accuracy protein-structure predictions, powered by DeepMind's AlphaFold v2.0. It provides programmatic access to a vast number of predicted structures and is expanding to cover more sequences.

NUCLEIC ACIDS RESEARCH (2022)

Article Biotechnology & Applied Microbiology

Engineering Novel (R)-Selective Transaminase for Efficient Symmetric Synthesis of d-Alanine

Dong-Xu Jia et al.

Summary: This study identified and improved an aminotransferase with high activity and thermostability towards pyruvate for the synthesis of d-alanine, achieving a high conversion yield.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2022)

Review Biotechnology & Applied Microbiology

Lactulose production from lactose isomerization by chemo-catalysts and enzymes: Current status and future perspectives

Mingming Wang et al.

Summary: Lactulose, a versatile semisynthetic nondigestive disaccharide, has gained increasing interest in the food and pharmaceutical industries for its health-promoting effects. Industrial production of lactulose is currently achieved through chemical isomerization of lactose, but enzymatic production offers a potentially greener alternative. Cellobiose 2-epimerase has emerged as an efficient enzyme for lactulose production through isomerization. This review summarizes recent developments in the chemical and enzymatic production of lactulose, as well as techniques for improving biocatalytic performance.

BIOTECHNOLOGY ADVANCES (2022)

Article Biochemistry & Molecular Biology

Enhancing the Catalytic Activity of Type II L-Asparaginase from Bacillus licheniformis through Semi-Rational Design

Yawen Zhou et al.

Summary: In this study, smart libraries were constructed using semi-rational design to improve the catalytic activity of type II L-asparaginase from Bacillus licheniformis. Mutants with significantly enhanced catalytic efficiency were obtained through saturation mutations and combinatorial mutations. The quintuple mutant ILRAC showed the highest specific activity and k(cat)/K-m, and also exhibited improved pH stability and thermal stability. Structural alignment and molecular dynamic simulation revealed that the stability and substrate binding of ILRAC contributed to its enhanced thermal stability and enzymatic activity. This study presents a novel semi-rational approach to enhance the enzymatic activity of type II L-asparaginase and provides insights for industrial applications.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Crystallography

Improving Thermostability of GH11 Xylanase XynASP by the Design of Loop Region

Tongbiao Li et al.

Summary: This study investigates the effect of mutations in the loop region on the thermal stability of GH11 xylanase. The results show that certain mutations can significantly enhance the thermostability of the enzyme, and the mechanisms for improved stability are elucidated through structural analysis.

CRYSTALS (2022)

Article Agriculture, Multidisciplinary

Significantly Improving the Thermostability and Catalytic Efficiency of Streptomyces mobaraenesis Transglutaminase through Combined Rational Design

Xinglong Wang et al.

Summary: This study significantly improved the catalytic properties of transglutaminase TGm1 by performing virtual proline scan and saturation mutagenesis to obtain variants FRAPD-TGm2 and FRAPD-TGm3 with enhanced stability and activity. These variants showed increased half-life, melting temperature, and specific activity compared to TGm1, making them robust candidates for transglutaminase application.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)

Article Biochemical Research Methods

A base measure of precision for protein stability predictors: structural sensitivity

Octav Caldararu et al.

Summary: The study analyzed the structural sensitivity of stability prediction methods for proteins and found that the methods can be grouped into two categories with varying sensitivity. Accuracy correlates with precision for mutation-type-balanced data sets, highlighting the importance of balance in mutation types. Machine-learning methods may underestimate the significance of protein structure compared to side-chain-sensitive methods.

BMC BIOINFORMATICS (2021)

Article Agriculture, Multidisciplinary

Enhancement of the Isomerization Activity and Thermostability of Cellobiose 2-Epimerase from Caldicellulosiruptor saccharolyticus by Exchange of a Flexible Loop

Lu Wang et al.

Summary: This study aimed to optimize the catalytic performances of CEs by flexible loop exchange, resulting in mutants with improved isomerization activity and thermostability. Molecular dynamics simulations showed that loop exchange enlarged the entrance of the active site for substrate binding and benefited proton transfer. This work demonstrated the importance of the flexible loop surrounding the active site in determining the isomerization activity and thermostability of CEs.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)

Article Multidisciplinary Sciences

Highly accurate protein structure prediction with AlphaFold

John Jumper et al.

Summary: Proteins are essential for life, and accurate prediction of their structures is a crucial research problem. Current experimental methods are time-consuming, highlighting the need for accurate computational approaches to address the gap in structural coverage. Despite recent progress, existing methods fall short of atomic accuracy in protein structure prediction.

NATURE (2021)

Article Biotechnology & Applied Microbiology

Biochemical characterization of two cellobiose 2-epimerases and application for efficient production of lactulose and epilactose

John Kristian Jameson et al.

Summary: Lactose, the most abundant by-product of the dairy industry, can be converted into valuable prebiotics lactulose and epilactose using cellobiose 2-epimerases (CEases). In this study, two CEases from thermophilic and mesophilic microorganisms were characterized. The optimal conditions for lactose conversion and product yields varied between the two enzymes at different temperatures.

CURRENT RESEARCH IN BIOTECHNOLOGY (2021)

Review Biotechnology & Applied Microbiology

Recent advances in the improvement of enzyme thermostability by structure modification

Zhe Xu et al.

CRITICAL REVIEWS IN BIOTECHNOLOGY (2020)

Article Biochemistry & Molecular Biology

Improving the thermostability and activity of Paenibacillus pasadenensis chitinase through semi-rational design

Pei Xu et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Article Biochemistry & Molecular Biology

Enhancing the thermostability of Rhizopus chinensis lipase by rational design and MD simulations

Rui Wang et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Article Biotechnology & Applied Microbiology

Computation-aided engineering of starch-debranching pullulanase fromBacillus thermoleovoransfor enhanced thermostability

Jiahua Bi et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

Article Biochemistry & Molecular Biology

Rational design of a Yarrowia lipolytica derived lipase for improved thermostability

Huitu Zhang et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Article Biochemistry & Molecular Biology

Simulation-guided enzyme discovery: A new microbial source of cellobiose 2-epimerase

Yaqin Xiao et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Article Biotechnology & Applied Microbiology

Consensus design for improved thermostability of lipoxygenase from Anabaena sp PCC 7120

Hui Qian et al.

BMC BIOTECHNOLOGY (2018)

Review Food Science & Technology

Lactulose: Properties, techno-functional food applications, and food grade delivery system

Majid Nooshkam et al.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2018)

Article Agriculture, Multidisciplinary

Characterisation of a novel cellobiose 2-epimerase from thermophilic Caldicellulosiruptor obsidiansis for lactulose production

Qiuming Chen et al.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2017)

Article Biochemistry & Molecular Biology

Improvement of thermostability and halostability of β-1,3-1,4-glucanase by substituting hydrophobic residue for Lys48

Jong Min Lee et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2017)

Article Biotechnology & Applied Microbiology

Development of a continuous membrane reactor process for enzyme-catalyzed lactulose synthesis

Azis Boing Sitanggang et al.

BIOCHEMICAL ENGINEERING JOURNAL (2016)

Article Agriculture, Dairy & Animal Science

Enzymatic production of lactulose and epilactose in milk

Eva Rentschler et al.

JOURNAL OF DAIRY SCIENCE (2015)

Article Biochemistry & Molecular Biology

Thermostability enhancement of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus by site-directed mutagenesis

Qiuyun Shen et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2015)

Article Multidisciplinary Sciences

Assessing Predictors of Changes in Protein Stability upon Mutation Using Self-Consistency

Grant Thiltgen et al.

PLOS ONE (2012)

Article Chemistry, Multidisciplinary

Creation of an Amino Acid Network of Structurally Coupled Residues in the Directed Evolution of a Thermostable Enzyme

Manfred T. Reetz et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)

Article Biochemistry & Molecular Biology

Enhanced thermostability and tolerance of high substrate concentration of an esterase by directed evolution

JH Kim et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2004)