Electrochemical immunosensing by carbon ink/carbon dot/ZnO-labeled-Ag@polypyrrole composite biomarker for CA-125 ovarian cancer detection

In this work, we demonstrated a novel cancer antigen 125 (CA125) biomarker detection based on electro-chemical immunosensor. The biomarker on conductive composite materials of carbon ink/carbon dot/zine oxide (C-ink/CD/ZnO) was employed as an electrode platform by using ITO substrate to enhance the interaction of antibodies (Ab) with supporting catalytic performance of ZnO as a labeling signal molecule. They were a scientist attention for biosensor with chemical stability, strong biocompatibility, high conductive signal, and accuracy. Moreover, the nanocomposite of silver@polypyrrole (Ag@PPy) was used as a potential redox mediator. The labeled construction with Ag@PPy was more accuracy than that of a free-labeled. The created immunosensor was a wide linear range as 1 ag center dot mL-1 -100 ng center dot mL-1 and a low limitation of detection as 0.1 fg center dot mL-1 under the optimal condition. This suggested that the immunosensor is considered to be an accurate and efficient diagnostic tool for CA125 and other biomarkers detection in actual sample analysis for clinic.

Automated summary

In this study, a novel cancer antigen 125 (CA125) biomarker detection method based on electro-chemical immunosensor was demonstrated. The biomarker on conductive composite materials of carbon ink/carbon dot/zinc oxide (C-ink/CD/ZnO) was used as an electrode platform, enhancing the interaction of antibodies (Ab) with the catalytic performance of ZnO. The created immunosensor showed a wide linear range and low detection limit, making it an accurate and efficient diagnostic tool for CA125 and other biomarkers in clinical sample analysis.