Lumichrome from the photolytic riboflavin acts as an electron shuttle in microbial photoelectrochemical systems

Riboflavin has been proposed to serve as an electron shuttle in photoelectrochemical systems. However, ribo-flavin was also observed for abiotic photolysis under illumination. Such conflicting reports raise the necessity for further investigation. In this study, riboflavin secreted by Rhodopseudomonas palustris was studied to clarify its stability and electron shuttle function under illumination. The data of high-performance liquid chromatogra-phy-mass spectrometry showed that the riboflavin was photolyzed to lumichrome in microbial photo-electrochemical systems. In addition, the anodic current increased by 75% after adding lumichrome compared with that of the control; it further demonstrated that lumichrome, not riboflavin, as an electron shuttle could facilitate microbial electron transfer. This study clarifies the mechanism of the interface process in microbial photoelectrochemical systems.

Automated summary

This study clarifies the mechanism of the interface process in microbial photoelectrochemical systems. High-performance liquid chromatography-mass spectrometry analysis showed that riboflavin secreted by Rhodopseudomonas palustris was photolyzed to lumichrome. Additionally, the anodic current increased by 75% after adding lumichrome, demonstrating that lumichrome, as an electron shuttle, facilitates microbial electron transfer.