Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 665, Issue -, Pages 169-177Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2023.04.108
Keywords
Genetically encoded calcium indicators; Green fluorescent ratiometric FNCaMP; indicator; Calcium imaging; Crystal structure; Chromophore
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Neurobiologists commonly use green genetically encoded calcium indicators (GECIs) to visualize neuronal activity. Ratiometric GECIs are less popular due to their inferior properties compared to intensiometric GCaMP GECIs. To address this issue, we engineered a ratiometric green calcium indicator called FNCaMP and compared its properties with FGCaMP7.
Neurobiologists widely use green genetically encoded calcium indicators (GECIs) for visualization of neuronal activity. Among them, ratiometric GECIs allow imaging of both active and non-active neuronal populations. However, they are not popular, since their properties are inferior to intensiometric GCaMP series of GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. However, the dynamic range and sensitivity of its large Stock's shift green (L SS-Green) form is significantly lower than its Green form and its molecular design is not optimal. To address these drawbacks, we engineered a ratiometric green calcium indicator, called FNCaMP, which is based on bright mNeonGreen protein and calmodulin from A. niger and has optimal NTnC-like design. We compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed X-ray structure of the FNCaMP indicator.(c) 2023 Elsevier Inc. All rights reserved.
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