Stemness targeting of colorectal cell lines mediated by BAMLET and 5-Flourouracil

Purpose: Stemness is the potential for self-renewal and repopulation causing the relapse, progression, and drug resistance of colorectal cancer. We investigated the effects of bovine alpha-lactalbumin made lethal to tumor cells and 5-Flourouracil consisting of bovine a-lactalbumin protein and oleic acid, on colorectal cancer cells on stemness.Methods: The quantitative real-time polymerase chain reaction assessed the expression levels of stemness-related genes (c-myc, Lgr5, OCT4). Expression of stemness-related surface markers (CD44 and CD24) was also measured by the flow cytometry technique following the treatments.Results: Our results indicated decreased expression levels of C-Myc, Lgr5, oct4 as the stemness-related genes (P < 0.0001), and reduced population of CD44 thorn as the stemness-related cell surface marker upon treatment with BAMLET and 5-Flourouracil. BAMLET inhibited the stemness more effectively than 5-Flourouracil (P < 0.0001). Conclusion: Based on the result, inhibition of the Stemness related-genes (C-Myc, Lgr5, Oct4) and the surface markers (CD 24 thorn and CD44 thorn ) is a promising therapeutic approach using BAMLET.(c) 2023 Elsevier Inc. All rights reserved.

Automated summary

This study investigated the effects of bovine alpha-lactalbumin made lethal to tumor cells and 5-Flourouracil on the stemness of colorectal cancer cells. The results showed that BAMLET and 5-Flourouracil treatment led to decreased expression levels of stemness-related genes and surface markers, with BAMLET being more effective than 5-Flourouracil in inhibiting stemness.