BMP-2-mediated signaling suppresses salivary gland development

Research regarding the process of salivary gland development and elucidation of related mechanisms are considered essential for development of effective treatments for conditions associated with salivary disease. Various reports regarding the effects of bone morphogenetic protein (BMP)-2 on hard tissue cells have been presented, though few have examined those related to salivary gland formation. Using an organ culture system, the present study was conducted to investigate the function of BMP-2 in salivary gland formation. Salivary glands obtained from embryonic day 13.5 mice and treated with BMP-2 showed suppression of primordial cell differ-entiation and also gland formation in a concentration-dependent manner. Furthermore, gland formation inhi-bition was suppressed by concurrent treatment with dorsomorphin, an inhibitor of the Smad pathway. Expression levels of AQP5, a marker gene for acinar cells, and Prol1, an opiorphin expressed in the lacrimal gland, were decreased in salivary glands treated with BMP-2. The present findings indicate that suppression of salivary gland formation, especially acinar differentiation, is induced by BMP-2, a phenomenon considered to be related to the Smad pathway.

Automated summary

Research on salivary gland development and related mechanisms is crucial for the treatment of salivary diseases. This study investigated the role of BMP-2 in salivary gland formation using an organ culture system. The results suggest that BMP-2 suppresses salivary gland formation, particularly acinar differentiation, through the Smad pathway.