Rearing is critical for forming spatial representations in pre-weanling rats

Rearing, i.e., standing on the hind limbs in an upright posture, is part of a rat's innate exploratory motor pro-gram. Here, we examined in developing rats whether rearing is critical for the pup's capability to form spatial representations based on distal environmental cues. Pups (male) were tested at PD18, i.e., the first day they typically exhibit stable rearing, on a spatial habituation paradigm comprising a Familiarization session (with the pup exposed to an arena with a specific configuration of distal cues) followed, 3 h later, by a Test session where the pups were either re-exposed to the identical distal cue configuration (NoChange) or a changed configuration (DistalChange). In Experiment 1, rearing activity (rearing events, duration) decreased from Familiarization to Test in the NoChange pups but, remained elevated in the DistalChange group indicating that these pups recognized the distal novelty. Recognition of distal novelty was associated with increased c-Fos expression in hippocampal and medial prefrontal cortex (mPFC) areas, compared with NoChange pups. Analysis of GAD67+ cells suggested a parallel increase in excitation and inhibition specifically in prelimbic mPFC networks in response to distal cue changes. In Experiment 2, the pups were mechanically prevented from rearing while still seeing the distal cues during Familiarization. Rearing activity in the Test session of these pups did not differ between groups that were or were not exposed to a changed distal cue configuration at Test. The findings evi-dence a critical role of rearing for the emergence of allocentric representations integrating distal space during early development.

Automated summary

This study examined the role of rearing in the emergence of spatial representations based on distal cues in developing rats. The results demonstrated that recognition of distal novelty was associated with increased c-Fos expression in the hippocampus and medial prefrontal cortex. Additionally, the findings suggested that rearing plays a critical role in the early development of allocentric representations integrating distal space.