4.7 Article

An in situ Raman spectroscopy-based microfluidic lab-on-a-chip platform for non-destructive and continuous characterization of Pseudomonas aeruginosa biofilms

Journal

CHEMICAL COMMUNICATIONS
Volume 51, Issue 43, Pages 8966-8969

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc02744f

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Funding

  1. National Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2014-05487]
  2. National Institute of Allergy and Infectious Diseases of the National Institutes of Health (NIH) [R21AI098701]
  3. Canadian Institutes for Health Research [MOP-74493]
  4. CSC (China)
  5. Fundacion la Caixa
  6. Fundacion Canada (Spain)

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Pseudomonas aeruginosa biofilm was cultivated and characterized in a microfluidic lab-on-a-chip platform coupled with confocal Raman microscopy in a non-destructive manner. Biofilm formation could be quantified by this label-free platformand correlated well with confocal laser scanning microscopy. This Raman-microfluidic platform could also discriminate biofilms at different developmental stages.

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