Journal
ARCHIVES OF ORAL BIOLOGY
Volume 155, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2023.105790
Keywords
Long noncoding RNA; Invasion; Metastasis; MAL; Oral cancer
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This study investigates the role of lncRNA-AC103563.8 in promoting oral squamous cell carcinoma (OSCC) development and explores its mechanism. The upregulation of lncRNA-AC103563.8 in OSCC tissue is observed, and it is found to promote cell invasion and metastasis. The study also identifies proteins that directly bind to lncRNA-AC103563.8 and explores their involvement in various biological processes.
Objectives: This study aims to investigate the role of the long non-coding RNA-AC103563.8 (lncRNA) in pro-moting oral squamous cell carcinoma (OSCC) development and to conduct preliminary research on its mechanism.Design: Microarray technology were used to screen out a lncRNA significantly upregulated in OSCC. Fluorescence in situ hybridization was used to analyze the position of lncRNA-AC103563.8 in cells. A Cal-27 cell line with knockout of the lncRNA-AC103563.8 gene was constructed. Transwell assay and tumor xenograft experiment was used to determine the metastasis and invasion of the cell. Detection of mutations in genes encoding myelin and lymphocyte proteins (MAL) by pyrosequencing. Identification of RNA-Binding Proteins by Mass Spectrom-etry (ChIRP-MS) experiments were carried out to enrich the proteins that directly bind to lncRNA-AC103563.8. Bioinformatics was used to analyze the target proteins. Some of the selected proteins were verified by parallel reaction monitoring (PRM) to confirm their binding to lncRNA-AC103563.8.Results: lncRNA-AC103563.8 is upregulated in OSCC tissue and the presence of lncRNA-AC103563.8 in both the nucleus and the cytoplasm. lncRNA-AC103563.8 promoted OSCC cell invasion and metastasis. Methylation occurs in MAL gene promoter. ChIRP-MS identified 330 proteins binding to lncRNA-AC103563.8, and bioin-formatics analysis showed that they were involved in a variety of biological processes. PRM experiments confirmed some protein directly bound to lncRNA-AC103563.8.Conclusion: lncRNA-AC103563.8 is a functional lncRNA that promotes OSCC development by acting on MAL or interacting with other tumor-related proteins. This study also indicates that this lncRNA may exert regulatory functions in OSCC and is a potential target for OSCC therapy.
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