Biosynthesis of resveratrol by an endophytic Priestia megaterium PH3 via the phenylpropane pathway

Resveratrol (RES) is a secondary metabolite synthesized by plants in response to environmental stress and pathogen infection, which is of great significance for the industrial production of RES by fermentation culture. In this study, we aimed to explore the biosynthesis pathway of RES and its key enzymes in the Priestia megaterium PH3, which was isolated and screened from peanut fruit. Through Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, we quantified the RES content and distribution in the culture medium and determined that Priestia megaterium PH3 mainly secreted RES extracellularly. Furthermore, the highest production of RES was observed in YPD, yielding an impressive 127.46 +/- 6.11 p.g/L. By optimizing the fermentation conditions, we achieved a remarkable RES yield of 946.82 +/- 24.74 p.g/L within just 2 days, which represents the highest reported yield for a natural isolate produced in such a short time frame. Our investigation revealed that the phenylpropane pathway is responsible for RES synthesis in this bacterium, with cinnamate 4-hydroxylase (C4H) identified as the main rate-limiting enzyme. Overall, our findings highlight the robust RES production capabilities of Priestia megaterium PH3, offering novel insights and potential applications for bacterial fermentation in RES production. Key points center dot RES synthesized by the bacterium was confirmed through the phenylpropane pathway.center dot The key rate-limiting enzyme for biosynthesis-RES is C4H.center dot RES reached 946.82 +/- 24.74 mu g/L after fermentation for 2 days.

Automated summary

In this study, the biosynthesis pathway and key enzymes of RES in Priestia megaterium PH3 were investigated. The highest RES production was achieved within a short time frame by optimizing fermentation conditions. This research provides valuable information and potential applications for RES production through bacterial fermentation.