Synergistic biocontrol of Bacillus subtilis and Pseudomonas fluorescens against early blight disease in tomato

Early blight of tomato caused by Alternaria solani results in significant crop losses. In this study, Bacillus subtilis J3 and Pseudomonas fluorescens J8 were co-cultured as a synthetic microbial community (BCA) for synergistic biocontrol of A. solani, and the inhibition mechanism was investigated. BCA presented an inhibition ration against A. solani at 94.91%, which lowered the disease incidence by 38.26-42.87%; reduced peroxidase, catalase, superoxide dismutase activity of tomatoes by 73.11-90.22%; and promoted the biomass by 66.91-489.21%. With BCA protection, the relative expression of tomato resistance genes (including gPAL2, SWRKY, PR-10, and CHI) in roots and leaves was 12.83-90.70% lower than without protection. BCA also significantly altered the rhizosphere and phyllosphere microbial community. The abundance of potentially beneficial bacteria, including Bacillus, Pseudomonas, Arthrobacter, Lysobacter, and Rhizobium, elevated by 6.58-192.77%. They were negatively correlated with resistance gene expression, indicating their vital involvement in disease control. These results provided essential information on the synergistic biocontrol mechanism of bacteria against pathogens, which could contribute to developing novel biocontrol strategies.{GRAPHIACAL ABSTRACT}

Automated summary

In this study, a synthetic microbial community BCA (consisting of Bacillus subtilis J3 and Pseudomonas fluorescens J8) was used to control the tomato early blight pathogen Alternaria solani. BCA effectively inhibited A. solani, reduced disease incidence, and increased tomato biomass. BCA also had a significant impact on the rhizosphere and phyllosphere microbial community. These findings provide important insights into the development of novel biocontrol strategies.