Journal
CHEMICAL COMMUNICATIONS
Volume 51, Issue 77, Pages 14443-14446Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc05337d
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Funding
- National Natural Science Foundation of China [21475116, 21275124]
- Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)
- Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1410]
- University Natural Science Foundation of Jiangsu Province [13KJB150039]
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A new label-free chemiluminescence (CL) immunoassay method is proposed for the cheap, convenient and sensitive detection of proteins through the co-immobilization of a capture antibody and horseradish peroxidase on a sensing interface. The specific immunoreaction on the interface effectively inhibits the enzymatic CL reaction, thus causing a decrease in CL signals.
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