Solution-State 2D NMR Spectroscopy of Mixtures HyperpolarizedUsing Optically Polarized Crystals

The HYPNOESYS method (Hyperpolarized NOE System), which relies on the dissolution of optically polarized crystals, has recently emerged as a promising approach to enhance the sensitivity of NMR spectroscopy in the solution state. However, HYPNOESYS is a single-shot method that is not generally compatible with multidimensional NMR. Here we show that 2D NMR spectra can be obtained from HYPNOESYS-polarized samples, using single-scan acquisition methods. The approach is illustrated with a mixture of terpene molecules and a benchtop NMR spectrometer, paving the way to a sensitive, information-rich and affordable analytical method. HYPNOESYS (Hyperpolarized NOE System) has recently emerged as a promising approach to enhance the sensitivity of NMR spectroscopy in the solution state. We show that 2D NMR spectra can be obtained from HYPNOESYS-polarized samples, using single-scan acquisition methods, paving the way to a sensitive, information-rich and affordable analytical method.+image

Automated summary

The HYPNOESYS method, relying on the dissolution of optically polarized crystals, enhances the sensitivity of NMR spectroscopy in the solution state. This study demonstrates the acquisition of 2D NMR spectra from HYPNOESYS-polarized samples using single-scan methods, paving the way for a sensitive, information-rich, and affordable analytical technique.