Decorated DNA-Based Scaffolds as Lateral Flow Biosensors

Here we develop Lateral Flow Assays (LFAs) that employ as functional elements DNA-based structures decorated with reporter tags and recognition elements. We have rationally re-engineered tile-based DNA tubular structures that can act as scaffolds and can be decorated with recognition elements of different nature (i.e. antigens, aptamers or proteins) and with orthogonal fluorescent dyes. As a proof-of-principle we have developed sandwich and competitive multiplex lateral flow platforms for the detection of several targets, ranging from small molecules (digoxigenin, Dig and dinitrophenol, DNP), to antibodies (Anti-Dig, Anti-DNP and Anti-MUC1/EGFR bispecific antibodies) and proteins (thrombin). Coupling the advantages of functional DNA-based scaffolds together with the simplicity of LFAs, our approach offers the opportunity to detect a wide range of targets with nanomolar sensitivity and high specificity.

Automated summary

In this study, we have developed Lateral Flow Assays (LFAs) using DNA-based structures decorated with reporter tags and recognition elements as functional elements. By re-engineering tile-based DNA tubular structures, we can decorate them with recognition elements of different nature and orthogonal fluorescent dyes, enabling the detection of a wide range of targets with nanomolar sensitivity and high specificity using sandwich and competitive multiplex lateral flow platforms.