Antibody-Conjugated Nanodiamonds as Dual-Functional Immunosensors for In Vitro Diagnostics

Article Chemistry, Multidisciplinary

SARS-CoV-2 Quantum Sensor Based on Nitrogen-Vacancy Centers in Diamond

Changhao Li et al.

Summary: A molecular transducer based on NV centers has been proposed to convert SARS-CoV-2 RNA into magnetic noise signal for optical readout. This sensor demonstrates high sensitivity, low false negative rate, and fast detection of the virus.

NANO LETTERS (2022)

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Review Chemistry, Analytical

Diagnoses Based on C-Reactive Protein Point-of-Care Tests

Miroslav Pohanka

Summary: C-reactive protein is an important component of the immune system's response to pathological impulses and can be used for diagnosing various pathological states. The current methods for CRP diagnosis have limitations, but research on biosensors and bioassay devices is providing more sensitive and suitable tools.

BIOSENSORS-BASEL (2022)

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Article Immunology

SARS-CoV-2 antibody progression and neutralizing potential in mild symptomatic COVID-19 patients - a comparative long term post-infection study

Jessica Brehm et al.

Summary: This study compared five serological tests on a cohort of 13 patients with mild COVID-19 disease. The results showed that individual immune responses remained stable over 455 days after a mild SARS-CoV-2 infection, while vaccinated participants showed different responses. All serological tests performed comparably and were well compared to a surrogate neutralization test. The information presented will assist clinicians in selecting and evaluating different serological tests in their daily laboratory work.

FRONTIERS IN IMMUNOLOGY (2022)

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Article Chemistry, Analytical

Fluorescent nanodiamond-based spin-enhanced lateral flow immunoassay for detection of SARS-CoV-2 nucleocapsid protein and spike protein from different variants

Wesley Wei -Wen Hsiao et al.

Summary: This report presents a novel detection platform called SELFIA for rapid testing of different variants of SARS-CoV-2 virus. The platform shows comparable accuracy and sensitivity with traditional testing methods.

ANALYTICA CHIMICA ACTA (2022)

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Article Chemistry, Analytical

Spin-Enhanced Lateral Flow Immunoassay for High-Sensitivity Detection of Nonstructural Protein NS1 Serotypes of the Dengue Virus

Trong-Nghia Le et al.

Summary: Dengue fever is a global viral infectious disease, and early detection is crucial for disease prevention and clinical care. In this study, a novel rapid and highly sensitive tool for dengue detection was developed, which can differentiate different NS1 protein serotypes, potentially enabling early detection in high-risk and limited-resource areas.

ANALYTICAL CHEMISTRY (2022)

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Article Nanoscience & Nanotechnology

Carboxylated/Oxidized Diamond Nanoparticles for Quantifying Immunoglobulin G Antibodies Using Mass Spectrometry

Avinash A. Patil et al.

Summary: Antibody-conjugated nanodiamonds are gaining attention in biomedical research, with high affinity carboxylated/oxidized ND particles being used to directly quantify antibodies like IgG. This method shows potential for enhancing sensitivity in drug delivery and immunotherapy applications.

ACS APPLIED NANO MATERIALS (2021)

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Review Chemistry, Physical

Diagnostics for SARS-CoV-2 infections

Bhavesh D. Kevadiya et al.

Summary: The outbreak of SARS-CoV-2 causing COVID-19 has led to diverse and severe symptoms, making accurate diagnosis a key challenge. Rapid diagnosis and treatment measures are crucial in controlling the spread of the virus. The development of diagnostic tools, including nanomaterial-based ones, is essential in the fight against the pandemic.

NATURE MATERIALS (2021)

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Article Chemistry, Analytical

Magnetically Modulated Fluorescence of Nitrogen-Vacancy Centers in Nanodiamonds for Ultrasensitive Biomedical Analysis

Yuen Yung Hui et al.

Summary: This study investigates the unique magneto-optical properties of the negatively charged nitrogen-vacancy center in fluorescent nanodiamonds and demonstrates its practical application in biomedicine. Using a periodic, time-varying magnetic field and lock-in detection method, selective and sensitive detection of FNDs was achieved with detection limits of 2 x 10(4) particles/mm(2) and 1 fM. The utility and versatility of the technique were showcased through background-free detection of FNDs as reporters and selective quantification of FNDs in tissue digests for in vivo studies.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Numerical Modelling of the Optical Properties of Plasmonic and Latex Nanoparticles to Improve the Detection Limit of Immuno-Turbidimetric Assays

Giuliano Coletta et al.

Summary: Combining plasmonic (gold, silver) and latex nanoparticles can improve the performance of immuno-turbidimetric assays without compromising the measurement dynamic range, providing a successful strategy to enhance the detection limit and maintain all the advantages of homogeneous immuno-turbidimetric assays.

NANOMATERIALS (2021)

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Review Chemistry, Multidisciplinary

Toward Quantitative Bio-sensing with Nitrogen-Vacancy Center in Diamond

Tongtong Zhang et al.

Summary: The nitrogen-vacancy (NV) center is a promising quantum sensor for biomedical applications, with unique optical and spin properties that allow for quantitative measurement of nanoscale electromagnetic fields at room temperature. Its potential in molecular detection and imaging makes it one of the most versatile platforms for various life-science applications.

ACS SENSORS (2021)

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Article Medicine, General & Internal

Characterization of the Diagnostic Performance of a Novel COVID-19 PETIA in Comparison to Four Routine N-, S- and RBD-Antigen Based Immunoassays

Alexander Spaeth et al.

Summary: In 2019, a novel coronavirus emerged in Wuhan, China, leading to the global COVID-19 pandemic caused by SARS-CoV-2. The virus is transmitted through droplets, aerosols, and close contact with infected individuals. Various techniques have been developed to assess and quantify the specific antibody response to SARS-CoV-2 infection. These techniques showed good overall agreement and suitability for quantifying the immune response derived from SARS-CoV-2.

DIAGNOSTICS (2021)

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Article Biochemical Research Methods

Rapid and accurate agglutination-based testing for SARS-CoV-2 antibodies

Sally Esmail et al.

Summary: A rapid, accurate, and cost-effective serologic test for SARS-CoV-2 virus has been developed based on antibody-dependent agglutination of antigen-coated latex particles, with high specificity and sensitivity for detecting antibodies against the virus. The strength of the S-RBD antibody response measured by the assay correlated with the efficiency of plasma in blocking RBD binding to the ACE2 receptor, suggesting the potential for identifying individuals with virus-neutralizing antibodies. Additionally, a high percentage of patients had detectable antibodies on the day of a positive viral RNA test, indicating the potential complementary role of the agglutination antibody test in diagnosing SARS-CoV-2 infection.

CELL REPORTS METHODS (2021)

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Article Multidisciplinary Sciences