Potential-Resolved Electrochemiluminescence Multiplex Immunoassay for Florfenicol and Chloramphenicol in a Single Sample

The simultaneous detection of multiple antibiotic residues in food is of great significance for food safety. In this work, a novel dual-potential electrochemiluminescence (ECL) immunoassay was designed for the simultaneous detection of chloramphenicol and fluorfenicol residues in food. Ru@MOF was used as an anodic probe, and SnS2 QDs-PEI-Au-MoS2 was used as a cathodic probe. Notably, the coreactant for both luminophores was K2S2O8, avoiding interactions caused by different kinds of coreactants. Au nanoparticles functionalized with a nitrogen- and sulfur-doped graphene oxide-modified glassy carbon electrode to improve the electron transfer efficiency and provide a larger surface area for immobilization of antigen. The linear range for the detection of florfenicol was determined to be 0.1-1000 ng mL(-1) with a detection limit of 0.03 ng mL(-1), and the linear range for the detection of chloramphenicol was 0.01-1000 ng mL(-1) with a detection limit of 3.2 pg mL(-1) by recording the ECL responses at two different excitation potentials. The proposed immunoassay achieved a more stable recovery in the detection of actual samples and provided a new analytical method for the simultaneous detection of florfenicol and chloramphenicol residues with high sensitivity and specificity.

Automated summary

Simultaneous detection of multiple antibiotic residues in food is important for food safety. A novel dual-potential electrochemiluminescence immunoassay was developed for the simultaneous detection of chloramphenicol and florfenicol residues in food. The proposed method achieved high sensitivity and specificity, as well as good stability in the detection of actual samples.