Journal
ANALYTICAL CHEMISTRY
Volume 95, Issue 40, Pages 14833-14841Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.3c01368
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This study reports a fluorescent probe for detecting carbonic anhydrases (CAs), which demonstrates excellent selectivity, favorable biocompatibility, and desirable blood-brain barrier (BBB) penetration. The probe enables imaging of CA fluctuations in cells and mice, providing insights into the role of CA in disease pathogenesis.
Carbonic anhydrases (CAs) participate in various physiological and pathological activities by catalyzing the interconversion between carbon dioxide and bicarbonate ions. Under normal circumstances, they guarantee that the relevant biological reactions in our body occur within an appropriate time scale. Abnormal expression or activity alteration of CAs is closely related to the pathogenesis of diverse diseases. This work reports an inhibitor-directed fluorescent probe FMRs-CA for the detection of CAs. Excellent selectivity, favorable biocompatibility, and desirable blood-brain barrier (BBB) penetration endow the probe with the ability to image the fluctuation of CAs in cells and mice. We achieved in situ visualization of the increased CAs in hypoxic cells with this probe. Additionally, probe FMRs-CA was mainly enriched within the liver and gradually metabolized by the liver. With the help of FMRs-CA, the increase of CAs in epileptic mouse brains was revealed first from the perspective of imaging, providing the mechanism connection between abnormal CA expressions and epilepsy.
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