Dual-functional Nanomaterials Polyo-phenylenediamine and Ru-Au Complement Each Other to Construct an Electrochemical and Electrochemiluminescent Dual-Mode Aptamer Sensor for Sensitive Detection of Alternariol

To sensitively monitor trace amounts of alternariol (AOH)in fruits,a dual-mode aptamer sensor utilizing the dual-function nanomaterialPoPD/Ru-Au was developed. This sensor provides both electrochemical(EC) and electrochemiluminescence (ECL) signals, which can greatlyavoid the potential false positive of the traditional single signal,thus enhancing the accuracy and reliability of detection results.Polyo-phenylenediamine (PoPD), known for its favorable EC response,can also assist in enhancing the ECL behavior of Ru-Au. Furthermore,Ru-Au demonstrates excellent ECL performance and effectivelyactivates K2S2O8 to amplify the ECresponse of PoPD. The complementary effect of the two can effectivelyamplify the final detection signal. Additionally, the PoPD/Ru-Aunanomaterial exhibits excellent electrical conductivity, further enhancingthe EC and ECL response signals. The experimental results demonstratethat the EC detection range of AOH was 0.01-100 ng/mL, whilethe ECL detection range was 0.001-100 ng/mL, both exhibitinga satisfactory linear relationship. Therefore, the mutual verificationof the detection results can be highly realized, and the purpose ofavoiding wrong detection can be achieved.

Automated summary

A dual-mode aptamer sensor utilizing the dual-function nanomaterial PoPD/Ru-Au was developed to sensitively monitor trace amounts of alternariol (AOH) in fruits. This sensor provides both electrochemical (EC) and electrochemiluminescence (ECL) signals, which can greatly avoid false positives and enhance detection accuracy and reliability.