Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 415, Issue 25, Pages 6145-6153Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-023-04909-y
Keywords
Surface-enhanced Raman scattering; Glutathione; Ferroptosis; Nanoprobe
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Here, an excellent plasmonic nanoprobe was developed for real-time monitoring of intracellular GSH levels during ferroptosis, providing important theoretical support for elucidating the GSH-related metabolic mechanism of ferroptosis.
Ferroptosis is a non-apoptotic cell death regulated by iron-dependent lipid peroxidation. Glutathione (GSH), a key antioxidant against oxidative damage, is involved in one of the most important metabolic pathways of ferroptosis. Herein, an excellent plasmonic nanoprobe was developed for highly sensitive, in situ, dynamic real-time monitoring of intracellular GSH levels during ferroptosis. A nanoprobe was prepared by functionalizing gold nanoparticles (AuNPs) with the probe molecule crystal violet (CV). The fluctuation in the SERS signal intensity of CV via the competitive displacement reaction can be used to detect GSH. The advantages of the plasmonic nanoprobe including low-cost production techniques, outstanding stability and biocompatibility, high specificity and sensitivity towards GSH with a detection limit of 0.05 mu M. It enables real-time dynamic monitoring of GSH levels in living cells during erastin-induced ferroptosis. This approach is expected to provide important theoretical support for elucidating the GSH-related ferroptosis metabolic mechanism and advancing our understanding of ferroptosis-based cancer therapy.
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