Journal
ANALYTICA CHIMICA ACTA
Volume 1279, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2023.341844
Keywords
Aflatoxin B1; Aptasensor; Dual-mode detection; MnO2 nanoflowers
Categories
Ask authors/readers for more resources
In this study, a colorimetric and fluorescent dual-mode aptasensor based on MnO2 nanoflowers was developed for accurate and sensitive detection of AFB1. The proposed method showed promising results in the determination of AFB1 in milk samples and exhibited high detection sensitivity and accuracy.
Aflatoxin B1 (AFB1) with tremendous toxic effects has caused a serious threat to food security. Accurate quantification of AFB1 in food can effectively prevent the risk of human intake of AFB1. Herein, a colorimetric and fluorescent dual-mode aptasensor for accurate and sensitive detection of AFB1 has been developed based on MnO2 nanoflowers (MnO2NFs) for the first time. MnO2NFs could catalyze the oxidation of 3,3 ',5,5 '-tetrame-thylbenzidine (TMB) into blue oxidation product (TMBox) by H2O2, which would be used for visible detection of AFB1. Simultaneously, MnO2NFs can be served as a signal amplifier and reduced by ascorbic acid to generate lots of Mn2+ which would quench the fluorescence of calcein for fluorescent detection of AFB1. Both colorimetric and fluorescent methods have been successfully applied for determination of AFB1 in milk samples with satisfactory results. The proposed dual-mode detection method with high detection sensitivity and accuracy showed great promise for monitoring AFB1 in food.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available