Journal
ANALYTICA CHIMICA ACTA
Volume 1271, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2023.341413
Keywords
Quadruple signal amplification strategy; Dual mode detection; Two-dimensional MoS2@C hollow nanorods; DNA hexahedral Nanoframework
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Researchers have developed a self-powered biosensing platform for ultra-sensitive dual-mode detection of tumor suppressor microRNA-199a, using two-dimensional carbon-coated molybdenum disulfide (MoS2@C) hollow nanorods combined with nucleic acid amplification strategies and DNA hexahedral nanoframework. The platform showed a wide linear range (0.0001-100 pM) and a low detection limit (4.94 amol/L, S/N = 3) for microRNA-199a. The method was successfully applied to the detection of actual serum samples, providing a novel approach for accurate and sensitive detection of tumor markers.
Two-dimensional carbon-coated molybdenum disulfide (MoS2@C) hollow nanorods are combined with nucleic acid signal amplification strategies and DNA hexahedral nanoframework to construct a novel self-powered biosensing platform for ultra-sensitive dual-mode detection of tumor suppressor microRNA-199a. The nanomaterial is applied on carbon cloth and then modified with glucose oxidase or using as bioanode. A large number of double helix DNA chains are produced on bicathode by nucleic acid technologies including 3D DNA walker, hybrid chain reaction and DNA hexahedral nanoframework to adsorb methylene blue, producing high E-OCV signal. Methylene blue also is reduced and an increased RGB Blue value is observed. For microRNA-199a detection, the assay shows a extensive linear range of 0.0001-100 pM with a low detection limit of 4.94 amol/L (S/N = 3). The method has been applied to the detection of actual serum samples, providing a novel method for the accurate and sensitive detection of tumor markers.
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