Application of an integrative system (2D PLExHPLC-PDA) for bioactive compound extraction and online quantification: Advantages, validation, and considerations

Article Chemistry, Applied

Simultaneous extraction and analysis of apple pomace by gradient pressurized liquid extraction coupled in-line with solid-phase extraction and on-line with HPLC

Laise C. Da Silva et al.

Summary: To overcome the challenges of sample preparation for chromatographic analysis of apple pomace, a solvent gradient using Pressurized Liquid Extraction (PLE) was proposed, where the solvent changes gradually from water to ethanol. Different dynamic gradients, static time, and temperatures were evaluated and found to affect the yields of target analytes. The use of extraction solvent gradient improved extraction yields of compounds with different characteristics. Coupling solid-phase extraction in-line allowed separation of compounds into fractions, with furfural, HMF, and chlorogenic acid eluting gradually, while flavonoids were retained and eluted in later fractions. On-line analysis by HPLC provided real-time information and a 3D chromatogram of the sample.

FOOD CHEMISTRY (2023)

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Review Food Science & Technology

Beyond aroma: A review on advanced extraction processes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis) to produce phenolic acids and diterpenes

Rodrigo S. Pizani et al.

Summary: Rosemary and sage are rich in bioactive compounds that can be extracted and used in various industries. This article discusses the chemical and biological properties of these compounds and explores different extraction techniques and recovery technologies. It also highlights the importance of integrating these plants into modern biorefineries and emphasizes the need for further research on technical, scalable, economic, and environmental aspects.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2022)

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Review Chemistry, Analytical

Emerging challenges in the extraction, analysis and bioanalysis of cannabidiol and related compounds

Virginia Brighenti et al.

Summary: CBD is a bioactive compound isolated from cannabis plants and has various pharmaceutical properties, primarily used for treating seizure disorder in children. It is non-psychoactive unlike THC. The market for hemp plant material and related products has expanded rapidly in recent years, with a focus on developing reliable analytical methods for CBD analysis in various samples.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2021)

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Article Chemistry, Analytical

Comprehensive analysis of phenolic compounds from natural products: Integrating sample preparation and analysis

Juliane Vigano et al.

Summary: This study discusses a new system for comprehensive analysis of phenolic compounds in natural products, based on bidimensional separation of pressurized liquid extraction and in-line solid-phase extraction coupled with HPLC-PDA.

ANALYTICA CHIMICA ACTA (2021)

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Article Acoustics