Journal
ANALYTICA CHIMICA ACTA
Volume 1270, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2023.341238
Keywords
Cascade dynamic DNA network; Gold-magnetic nanochains; Signal amplification; SERS aptasensor; Tetracycline
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An efficient analytical method is needed to detect the misuse of tetracycline (TC) in animal husbandry and aquaculture, which poses a great threat to food and environmental safety. In this study, a cascade amplification SERS aptasensor was constructed for sensitive determination of TC using aptamer, enzyme-free DNA circuits, and SERS technology. Under optimal conditions, the developed aptasensor demonstrated a distinct linear response to TC with a low limit of detection of 15.91 pg mL-1. The proposed sensing strategy showed excellent specificity, storage stability, and practicality, providing a promising idea for the development of specific and sensitive signal amplification analysis platforms in the field of food safety.
Tetracycline (TC) poses a great threat to food and environmental safety due to its misuse in animal husbandry and aquaculture. Therefore, an efficient analytical method is needed for the detection of TC to prevent possible hazards. Herein, a cascade amplification SERS aptasensor for sensitive determination of TC was constructed based on aptamer, enzyme-free DNA circuits, and SERS technology. The capture probe and signal probe were obtained by binding DNA hairpins H1 and H2 to the prepared Fe3O4@hollow-TiO2/Au nanochains (Fe3O4@h-TiO2/Au NCs) and Au@4-MBA@Ag nanoparticles, respectively. The dual amplification of EDC-CHA circuits significantly facilitated the sensitivity of the aptasensor. Additionally, the introduction of Fe3O4 simplified the operation of the sensing platform due to its superb magnetic capability. Under optimal conditions, the developed aptasensor exhibited a distinct linear response to TC with a low limit of detection of 15.91 pg mL-1. Furthermore, the proposed cascaded amplification sensing strategy exhibited excellent specificity and storage stability, and its practicability and reliability were verified by TC detection of real samples. This study provides a promising idea for the development of specific and sensitive signal amplification analysis platforms in the field of food safety.
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